Throat swabs obtained from 100 children suspected for pneumonia(Group 1), from 200 children suspected for other respiratory diseases(Group 2), and from 100 adult suspected for respiratory diseases(Group 3) were examined for Mycoplasma pneumoniae
infection by culture and polymerase chain reaction(PCR). In 33 isolated M. pneumoniae strains, the susceptibilities to tetracycline, erythromycin, josamycin, sparfloxacin, ofloxacin, ciprofloxacin, and trimethoprim were determined by the broth
dilution
method.
The rate of isolation of M. pneumoniae by culture method was 12% for the first group, 6.5% for the second group, and 8% for the third group. The rates of detection of M. pneumoniae by PCR was 29% for the first group, 17% for the second group, and
14%
for the third group. The MIC for 90% inhibition of the isolated M. pneumoniae was 0.195μg/ml for sparfroxacin, 0.195 for erythromycin, 0.195 for josamycin, 0.78 for tetracycine, 30125 for ciprofloxacin, and 25.0 for trimethoprim.
These results indicate that 1) the PCR has a significant potential as a rapid and sensitive method for early detection of M. pneumoniae in clinical samples as compared with culture method and 2) sparfloxacin, erythromycin and josamycin could be
used as
antimycoplasmal agents.
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